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Generating diCre/LoxP based inducible gene knockout constructs for Leishmania

Please note that due to changes in the 3 fragment Gateway cloning reaction provided by Invitrogen our plasmids are no longer compatible. Instead, the Gateway vectors can be used to make the DiCre vectors by amplifying the relevant sections (and complementary overhangs) by PCR and assembling using Gibson Assembly.

Published Protocols

DiCre-Based Inducible Disruption of Leishmania Genes

DiCre-Based Inducible Gene Expression

Plasmids (Download .txt then rename to .gbk)

Find these plasmids on Addgene.

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