LOCUS pGL2314 5908 bp DNA circular UNA 01-APR-2009 DEFINITION plasmid for L. major ISP cluster KO PURr ACCESSION pGL1160 KEYWORDS 3` & 5` flanks; ampicillin resistance; hygromycin resistance; mexicana. SOURCE Expression vector pRIBOTEX Expression vector pRIBOTEX (japonica cultivar-group) ORGANISM Expression vector pRIBOTEX Expression vector pRIBOTEX artificial sequences; vectors FEATURES Location/Qualifiers primer 537..552 /label="M13 F" Gateway 570..668 /label="attL1" misc_feature 675..708 /feature_type="LoxP Site" /label="LoxP" misc_feature 709..895 /feature_type="Splice acceptor site" /label="pX SL" misc_feature 896..925 /feature_type="Insertion" /label="GOI Insertion Site" protein_bind 932..1096 /label="6xHA" misc_feature 1103..1136 /feature_type="LoxP Site" /label="LoxP" primer 1139..1162 /label="OL4065" polyA_site 1145..1640 primer complement(1283..1297) /label="OL2060" misc_signal 1641..1846 /label="Splice Acceptor Site" CDS 1847..2443 /label="PAC" Site join(<2457..>2460,<2461..2461,2456^2457) /site_type="restriction site" /note="Name: XhoI" /note="Pattern: ctcgag" /note="cut_0_on_positive_strand: 380^381" /note="cut_0_on_negative_strand: 384^385" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label="XhoI" misc_feature 2462..2649 /feature_type="Splice acceptor site" /label="SAS" CDS 2650..2735 /vntifkey="4" /label="HASPB N-term" primer complement(2727..2744) /label="OL4290" CDS 2753..3460 /vntifkey="21" /label="mCherry" Tag 3461..3478 /label="His Tag" Site join(3489..>3490,complement(3491..>3494),3490^3491,complement(3490^3491)) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="cut_0_on_positive_strand: 1516^1517" /note="cut_0_on_negative_strand: 1520^1521" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label="NotI" polyA_site 3500..4024 /label="Lmcpb Poly A" polyA_signal 3927..3941 /label="S1" Gateway 4033..4131 /label="attL2" primer complement(4173..4189) /label="M13 R" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTTA GATCATAACT TCGTATAGCA TACATTATAC GAAGTTATAA TTGCCCGCTT 721 TCCATTTCGT CACCTTCCGC CTCTCTCTCT CTCTCTCTCA CCATCTACGC GTGCACATCA 781 TCAACTGTCT CTTGTCGGTG CTCACCACCC TCAACCACCC CTCACTTTCA AGGCTTCCCG 841 AACGCACACA AAAGGCGTGA AAACCGCTCG CGTGTGTTGA GCCGTCCACC GTAGCCATAT 901 GGAATTCAGT ACTGGTACCA TCGATACTAG TTACCCTTAC GATGTGCCTG ATTACGCGTA 961 CCCATACGAC GTGCCAGACT ACGCATACCC GTACGATGTG CCCGATTACG CATACCCTTA 1021 CGATGTGCCT GATTACGCGT ACCCATACGA CGTGCCAGAC TACGCATACC CGTACGATGT 1081 GCCCGATTAC GCATGATCTA GAATAACTTC GTATAGCATA CATTATACGA AGTTATGGCT 1141 GCAGCGCGTG GATGTCGCGC AGCGCAAGAC CCCGTGCGCC TGAAACGTGA AGGGAGGTCG 1201 AGAAGCGTGC CGCATGAGGC CTTAAGGCAG GAAAGTGAAA AGAGCTCGAC GCGAGTGCAG 1261 CACGCGCGAC ATCGAACAGG ACGGAATAGA CACTCAGCCC CTTCCCCTTT CAAAAACTGA 1321 ATGGACGGAC ATCGTAACGC GCTTCTCTCC CCTCCACTCC CCCTGTCCCT GTCTCCTTGA 1381 CGTGTGCTGC TCTCGTGAGC GACAGATGAA GCGAGGCGTA GAAGGAAAGG GGGGGGAGCG 1441 AGGCCTGCAC ACACACACAC ACACACACAC ACACACACAC ACACACACAC ACACACACAC 1501 ACACGAAAAG CAAGTTTGAG GCAGACGTGG TTTCACACGA TTCCTGTGCA AGAAGCGTGC 1561 CTGCGTGACG GTGTGGCTGT GTATGCATGC GTGAAGGGTT ACCGCAACGA TGCGCAATGG 1621 ACTCCCCCGC TTTCCATTTC GTCACCTTCC GCCTCTCTCT CTCTCTCTCT CACCATCTAC 1681 GCGTGCACAT CATCAACTGT CTCTTGTCGG TGCTCACCAC CCTCAACCAC CCCTCACTTT 1741 CAAGGCTTCC CGAACGCACA CAAAAGGCGT GAAAACCGCT CGCGTGTGTT GAGCCGTCCA 1801 CCGTAGCCCT CCCCCTGTCC CCGGGGGATC CACTAGACGA CCTTCCATGA CCGAGTACAA 1861 GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCCGG GCCGTACGCA CCCTCGCCGC 1921 CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAC CCGGACCGCC ACATCGAGCG 1981 GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG 2041 GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG 2101 GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC 2161 GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT 2221 GGCCACCGTC GGCGTCTCGC CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT 2281 CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC 2341 CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGTGCCC 2401 GAAGGACCGC GCGACCTGGT GCATGACCCG CAAGCCCGGT GCCTAGGCTA GCGCTCTCGA 2461 GAATTGCCCG CTTTCCATTT CGTCACCTTC CGCCTCTCTC TCTCTCTCTC TCACCATCTA 2521 CGCGTGCACA TCATCAACTG TCTCTTGTCG GTGCTCACCA CCCTCAACCA CCCCTCACTT 2581 TCAAGGCTTC CCGAACGCAC ACAAAAGGCG TGAAAACCGC TCGCGGTGTG TTGAGCCGTC 2641 CACCGTAGCT GATCCTATCT ATCTCCCCCG CTTATACACC ATGGGAAGCT CTTGCACGAA 2701 GGACTCCGCA AAGGAGCCCC AGAAAAGTGC GGATGGATCT ATGGAGGATC CAATGGTTAG 2761 TAAAGGAGAA GAAAATAACA TGGCAATCAT TAAGGAGTTC ATGAGATTCA AAGTTCACAT 2821 GGAAGGTTCT GTAAATGGAC ATGAATTTGA AATAGAAGGT GAAGGAGAAG GAAGGCCTTA 2881 TGAAGGAACC CAAACCGCGA AGCTAAAAGT TACTAAGGGT GGCCCATTAC CATTTGCATG 2941 GGATATCCTT AGCCCTCAAT TCATGTATGG GTCAAAGGCT TATGTCAAGC ACCCCGCCGA 3001 CATTCCAGAC TATCTAAAGT TATCTTTTCC CGAAGGGTTT AAGTGGGAGC GTGTGATGAA 3061 CTTCGAAGAC GGTGGCGTGG TAACAGTGAC TCAGGATTCG TCCCTGCAAG ATGGTGAATT 3121 TATCTACAAA GTCAAATTAA GAGGAACTAA CTTTCCATCT GGCGGCCCGG TTATGCAAAA 3181 AAAGACAATG GGCTGGGAGG CCTCCTCAGA ACGAATGTAC CCTGAAGATG GTGCCTTGAA 3241 GGGTGAGATT AAACAAAGAT TGAAATTGAA AGATGGTGGA CATTATGACG CTGAGGTTAA 3301 AACGACATAC AAAGCTAAGA AACCTGTCCA GCTCCCAGGT GCTTACAATG TAAATATAAA 3361 ACTTGATATT ACATCACATA ATGAAGATTA TACGATAGTT GAACAATACG AAAGGGCTGA 3421 GGGGAGACAT AGTACTGGTG GCATGGATGA ACTATACAAA CATCATCATC ATCATCATTG 3481 AGGATCCCGC GGCCGCTAGA TGTGTGCCCT TGTGTGCGTG TGTGTTTGGT GGTTTGCAGC 3541 GATGCCCGGC GCGTGTGGGC ACCTCCTTGG GTGCGCGCCC GCCGTGGCAG CTGCGCGTGC 3601 GTGCGAGATG TGAGGCAGAG GAAGAGGAAG GCGATGCGGG CGACAGCGCA GCGAGGTGCG 3661 GCGGAGCGTA GGGGGGAAAT GGACGAGCAG GCGCGCTGTG AATCGGAGCT GCGGCACCAC 3721 CCAAGTCGTG GTGCCCCGCG AATGGCTGTT CTGCCCGCCC TCGCTTCACG CCTCCCCCTC 3781 CCCTCGCGTG CCCTCGCGTG GCCTCCCTTG TTATCCCTCT CTCTCGCACG CACACGCACA 3841 CGCGTATACG CGAGCCCGCT ATTCTGCCTT CGTCTGGCTC TTTGTATTTT GCTTGCTTCT 3901 TCAGCACACT TGTGTGCTGT GCGTTCAGCG ATATCTTCCA CTACTTTGTT TTCTCCTCCC 3961 CCTCGGGAGG TGCTTCGCTT GTGCTTTGAC GGTGGTGCGT GGCTGCTGGG TTATGTGCCG 4021 GGCGCCCATG GAACCCAGCT TTCTTGTACA AAGTTGGCAT TATAAGAAAG CATTGCTTAT 4081 CAATTTGTTG CAACGAACAG GTCACTATCA GTCAAAATAA AATCATTATT TGCCATCCAG 4141 CTGATATCCC CTATAGTGAG TCGTATTACA TGGTCATAGC TGTTTCCTGG CAGCTCTGGC 4201 CCGTGTCTCA AAATCTCTGA TGTTACATTG CACAAGATAA AAATATATCA TCATGAACAA 4261 TAAAACTGTC TGCTTACATA AACAGTAATA CAAGGGGTGT TATGAGCCAT ATTCAACGGG 4321 AAACGTCGAG GCCGCGATTA AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG 4381 CTCGCGATAA TGTCGGGCAA TCAGGTGCGA CAATCTATCG CTTGTATGGG AAGCCCGATG 4441 CGCCAGAGTT GTTTCTGAAA CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA 4501 TGGTCAGACT AAACTGGCTG ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC 4561 GTACTCCTGA TGATGCATGG TTACTCACCA CTGCGATCCC CGGAAAAACA GCATTCCAGG 4621 TATTAGAAGA ATATCCTGAT TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTTCCTGC 4681 GCCGGTTGCA TTCGATTCCT GTTTGTAATT GTCCTTTTAA CAGCGATCGC GTATTTCGTC 4741 TCGCTCAGGC GCAATCACGA ATGAATAACG GTTTGGTTGA TGCGAGTGAT TTTGATGACG 4801 AGCGTAATGG CTGGCCTGTT GAACAAGTCT GGAAAGAAAT GCATAAACTT TTGCCATTCT 4861 CACCGGATTC AGTCGTCACT CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG 4921 GGAAATTAAT AGGTTGTATT GATGTTGGAC GAGTCGGAAT CGCAGACCGA TACCAGGATC 4981 TTGCCATCCT ATGGAACTGC CTCGGTGAGT TTTCTCCTTC ATTACAGAAA CGGCTTTTTC 5041 AAAAATATGG TATTGATAAT CCTGATATGA ATAAATTGCA GTTTCATTTG ATGCTCGATG 5101 AGTTTTTCTA ATCAGAATTG GTTAATTGGT TGTAACACTG GCAGAGCATT ACGCTGACTT 5161 GACGGGACGG CGCAAGCTCA TGACCAAAAT CCCTTAACGT GAGTTACGCG TCGTTCCACT 5221 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG 5281 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC 5341 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA 5401 CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA 5461 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC 5521 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG 5581 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC 5641 AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG 5701 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT 5761 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT 5821 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG 5881 CCTTTTGCTG GCCTTTTGCT CACATGTT //