LOCUS pGL2313 6735 bp DNA circular UNA 05-Oct-2010 DEFINITION Invitrogen MS/GW donor vector 221 FEATURES Location/Qualifiers Gateway 570..668 /label="attL1" 5'UTR 679..873 /label="Actin 5' UTR" SAS 800^801 /label="Splice Leader Site" CDS 874..906 /cds_type="ORF" misc_feature 907..1218 /feature_type="Rapamycin Binding Domain" /label="FKBP12" primer complement(910..927) /label="OL4102" Linker 1219..1263 misc_feature 1258..1277 /feature_type="RT PCR Primer" /label="OL4691" misc_feature 1264..1389 /feature_type="Cre Recombinase Dimer" /label="Cre59" misc_feature complement(1315..1334) /feature_type="RT PCR Primer" /label="OL4692" 3'UTR 1390..2229 /label="Actin 3'UTR" Site join(1565..1570,1565^1566) /site_type="restriction site" /note="Name: BamHI" /note="Pattern: ggatcc" /note="cut_0_on_positive_strand: 1526^1527" /note="cut_0_on_negative_strand: 1530^1531" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label="BamHI" misc_feature 1571..2218 /feature_type="Insertion" /label="3'UTR Insertion site" misc_feature 2020^2021 /feature_type="PolyA Site" /label="PolyA Site" Site join(2219..2226,2220^2221) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="cut_0_on_positive_strand: 1547^1548" /note="cut_0_on_negative_strand: 1551^1552" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label="NotI" 5'UTR 2230..2427 /label="Beta Tubulin 5'UTR" SAS 2353 /label="Splice Leader Site" CDS 2428..2460 /label="ORF" misc_feature 2461..2737 /feature_type="Rapamycin Binding Domain" /label="FRB" Linker 2738..2772 misc_feature 2773..3627 /feature_type="Cre Recombinase Dimer" /label="Cre60" misc_feature 2796..2815 /feature_type="RT PCR Primer" /label="OL4693" misc_feature complement(2851..2870) /feature_type="RT PCR Primer" /label="OL4694" 3'UTR 3628..3825 /label="Beta Tubulin 3'UTR" 5'UTR 3847..4146 /label="BLAr 5'UTR" CDS 4147..4542 /label="BLAr" primer 4523..4540 /label="OL4101" 3'UTR 4543..4842 /label="BLAr 3'UTR" Gateway 4858..4958 /label="attL2" CDS 5129..5938 /label="Kanamycin Resistance" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTTG CCCTCGAGGT TTTCGTCGCG CATCGGTGTA GGTCGAATAC CCTTGCCTTT 721 CAGTGCGGGG TTTTTTCCTA TAAAGCATTT ATTGTCTCGA CGCTTCTCCT GCTTGGTTCT 781 CTCTCGCACT TGCATGACAG ATTTCAAGAT ATCTTGCCTG TACACTCGTC ATTAAAAACA 841 GCTTATTCCC GGTTATTTTT CTCAACGAAG AAAATGGCCC CTAAGAAGAA GAGAAAGGTG 901 TCAAGAGGAG TGCAGGTGGA AACCATCTCC CCAGGAGACG GGCGCACCTT CCCCAAGCGC 961 GGCCAGACCT GCGTGGTGCA CTACACCGGG ATGCTTGAAG ATGGAAAGAA ATTTGATTCC 1021 TCCAGGGACA GAAACAAGCC CTTTAAGTTT ATGCTAGGCA AGCAGGAGGT GATCCGAGGC 1081 TGGGAAGAAG GGGTTGCCCA GATGAGTGTG GGTCAGAGAG CCAAACTGAC TATATCTCCA 1141 GATTATGCCT ATGGTGCCAC TGGGCACCCA GGCATCATCC CACCACATGC CACTCTCGTC 1201 TTCGATGTGG AGCTTCTAAA ACTGGAAGCT AGCCCCAGCA ACCCCGGCGC CAGCAACGGC 1261 TCCACCTCTG ATGAAGTCAG GAAGAACCTG ATGGACATGT TCAGGGACAG GCAGGCCTTC 1321 TCTGAACACA CCTGGAAGAT GCTCCTGTCT GTGTGCAGAT CCTGGGCTGC CTGGTGCAAG 1381 CTGAACTGAA GGTCCTCACC CGACTGGGCA GCTGGAGTGC AGAGAACTTT GCGCGACCGG 1441 AAGACGCTAG TGAGATTGGG CACAGTGGAG CTAGCGTGCG CGCGGTAGCT TTGGCAGCAC 1501 TGGGATTTTG TCCTCTGTAC TGCCTGGCTC TCGTGTTTAT TTTTGGTTAG AGTGTGCGTT 1561 GCTCGGATCC CTGCCTGGTT CTCGTGCTTA TTTTTGGTTA GATGGTGCGT TGTTCTACTT 1621 TCAATCATCG TTTTGTTTTG TTTTTCATAT CTGTTTTCCA AAGAACTCTG ATTGTCTTCA 1681 TCCACCCGGT TTTCCCCGCC TGGTCTCGCT TTGAGATTGT TTTTATTCCT CTCCGGTTTT 1741 TCCCCATTGG GATAAAAGTG CGTCCCGTGT GTCTACCCCT CCAGTCCCCA CAAGCGGCTC 1801 AGATGTACAG AGCACCTGCT CTTCTCTGAC GTGCACTTTT TGCTCTTGCA GTCTTGCGCG 1861 GCCTCTAGTC TCCCCTGCCA CGCACGGATG CTGACGCCAA GCAGCATTCT GCGCAGGAAA 1921 GCGTGGTATC CCGACGAGGC AGATCGCGGG CTCGCATGTA CCGGCGACGG ACATCCTACA 1981 CACATTATCC TCATGTAGAA CCTCAAAGCA AGGGAAGAAG AAAAGGGTTG ACACGTTGTT 2041 ATGTTTTTTT TTGTGCCTGG GAGTAAGCGG GCGCCGATCT TCAAGTATCC TCTCAATTTC 2101 ACTGGCAGGG CGCGAAGTGC GCGGGCTCCT CGCGTGCCGC CGCAATGTCA GTCGCGGCTC 2161 TACCAGTGCT ACAGGGATGT CGGGTGGTGT CGTTGATGAT TTGGCATCAA GTGCACGCGC 2221 GGCCGCTTTC TTCCATCTGC ACTCGCTCTG CGCTTCGTTT TCTTCTTCCC TTGCGCGCGC 2281 GTGACGCCGC GCTCCTCACC TGCGCGCCCC GCCCCGACTT GGGTCCTCTC GTGCTGCAAC 2341 GGACGCCGAC AGCTTCCTTC CACGCATTTC GTGCCTCCGC ACCGTTCTCC ACCGCCAGCG 2401 CAGACTTGTA CATACGCACA CACCACCATG GCCCCTAAGA AGAAGAGAAA GGTGTCAAGA 2461 ATCCTCTGGC ATGAGATGTG GCATGAAGGC CTGGAAGAGG CATCTCGTTT GTACTTTGGG 2521 GAAAGGAACG TGAAAGGCAT GTTTGAGGTG CTGGAGCCCT TGCATGCTAT GATGGAACGG 2581 GGCCCCCAGA CTCTGAAGGA AACATCCTTT AATCAGGCCT ATGGTCGAGA TTTAATGGAG 2641 GCCCAAGAGT GGTGCAGGAA GTACATGAAA TCAGGGAATG TCAAGGACCT CCTCCAAGCC 2701 TGGGACCTCT ATTATCATGT GTTCCGACGA ATCTCAGCTA GCCCCAGCAA CCCCGGCGCC 2761 AGCAACGGCT CCAACAGGAA ATGGTTCCCT GCTGAACCTG AGGATGTGAG GGACTACCTC 2821 CTGTACCTGC AAGCCAGAGG CCTGGCTGTG AAGACCATCC AACAGCACCT GGGCCAGCTC 2881 AACATGCTGC ACAGGAGGTC TGGCCTGCCT CGCCCTTCTG ACTCCAATGC TGTGTCCCTG 2941 GTGATGAGGA GAATCAGAAA GGAGAATGTG GATGCTGGGG AGAGAGCCAA GCAGGCCCTG 3001 GCCTTTGAAC GCACTGACTT TGACCAAGTC AGATCCCTGA TGGAGAACTC TGACAGATGC 3061 CAGGACATCA GGAACCTGGC CTTCCTGGGC ATTGCCTACA ACACCCTGCT GCGCATTGCC 3121 GAAATTGCCA GAATCAGAGT GAAGGACATC TCCCGCACCG ATGGTGGGAG AATGCTGATC 3181 CACATTGGCA GGACCAAGAC CCTGGTGTCC ACAGCTGGTG TGGAGAAGGC CCTGTCCCTG 3241 GGGGTTACCA AGCTGGTGGA GAGATGGATC TCTGTGTCTG GTGTGGCTGA TGACCCCAAC 3301 AACTACCTGT TCTGCCGGGT CAGAAAGAAT GGTGTGGCTG CCCCTTCTGC CACCTCCCAA 3361 CTGTCCACCC GAGCCCTGGA AGGGATCTTT GAGGCCACCC ACCGCCTGAT CTATGGTGCC 3421 AAGGATGACT CTGGGCAGAG ATACCTGGCC TGGTCTGGCC ACTCTGCCAG AGTGGGTGCT 3481 GCCAGGGACA TGGCCAGGGC TGGTGTGTCC ATCCCTGAAA TCATGCAGGC TGGTGGCTGG 3541 ACCAATGTGA ACATTGTGAT GAACTACATC AGAAACCTGG ACTCTGAGAC TGGGGCCATG 3601 GTGAGGCTGC TAGAGGATGG GGACTGAAGT GTGCGTGAGT GTGCGCGTGC GTGTTGGTGC 3661 GCACCGCGCT CGCTGTCCCC GCGGCGCACG CACACCTCCC TCGCTCTCTC CCCCTCTTTC 3721 CGTGCCTTCT TTGTCTCCCT TCCTGTACGC TATTTTGGTT TCTTGTGCTT GCTTTATCTT 3781 GTGGGCGGGT GTGGGCCCGA CCTGCACGTC TGTGACGGAG TGACGTCTAG AGAGCTCGGG 3841 TCGACCACAC GTACACACGT ACACACACAC GTGCGCATGC TTGACGCATA CGCGACGAAT 3901 TCGAAAGCTC ACCTCATTCC TCCCTCCTCA CACCATCATC GGCATCCATA GAGACACGTG 3961 CGCGTAGAAC GATACAGCTC ACGCGCACAG AGAGGCGTGT TCTGGTCGTG CGCATCATCG 4021 GCACAGCACT GGCGGACGAA ACTCGCACAC AGGCACGCCG CCTCCTTTCA CCCGTCATAG 4081 ATAGTTGAAT TAGACGCCCT CCTCCTCCCT CATCATCGCC GTCGTCATCC GGGTCCGAGC 4141 ACTAGTATGG CCAAGCCTTT GTCTCAAGAA GAATCCACCC TCATTGAAAG AGCAACGGCT 4201 ACAATCAACA GCATCCCCAT CTCTGAAGAC TACAGCGTCG CCAGCGCAGC TCTCTCTAGC 4261 GACGGCCGCA TCTTCACTGG TGTCAATGTA TATCATTTTA CTGGGGGACC TTGTGCAGAA 4321 CTCGTGGTGC TGGGCACTGC TGCTGCTGCG GCAGCTGGCA ACCTGACTTG TATCGTCGCG 4381 ATCGGAAATG AGAACAGGGG CATCTTGAGC CCCTGCGGAC GGTGCCGACA GGTGCTTCTC 4441 GATCTGCATC CTGGGATCAA AGCCATAGTG AAGGACAGTG ATGGACAGCC GACGGCAGTT 4501 GGGATTCGTG AATTGCTGCC CTCTGGTTAT GTGTGGGAGG GCTAAAGATC TTCTAGTGAT 4561 CCCGCCCGCA CCCGCGCGCG CTCAGGCCGC GTGTCGTCGT GCTCTTTCCA TTTTTTTTTT 4621 GTGTGTGGTG GTGATTTGAG CTGCTCTCCG TTGTGTGCTG GGGACCCTCC TTCCCTCGAT 4681 CTCCTCGTGC GGGGTCTCCG ACGCGCAGAC GCGGTGCGTG CGAGCGTGCA CGTGCTGTCG 4741 CCGTGGCTGT AGTTGCGAGC GGAGAGAGAG AGAGAGGGAG GGAGGGAGGG CAGAGGGCAG 4801 AGGACATGCG GGTGGGAACG TGCACCGGCC TCGTCTCACG CAGGCCCGGG CGGTACCCTA 4861 CCCAGCTTTC TTGTACAAAG TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA 4921 CGAACAGGTC ACTATCAGTC AAAATAAAAT CATTATTTGC CATCCAGCTG ATATCCCCTA 4981 TAGTGAGTCG TATTACATGG TCATAGCTGT TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA 5041 TCTCTGATGT TACATTGCAC AAGATAAAAA TATATCATCA TGAACAATAA AACTGTCTGC 5101 TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT CAACGGGAAA CGTCGAGGCC 5161 GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT 5221 CGGGCAATCA GGTGCGACAA TCTATCGCTT GTATGGGAAG CCCGATGCGC CAGAGTTGTT 5281 TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA 5341 CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA 5401 TGCATGGTTA CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT TAGAAGAATA 5461 TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC 5521 GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA 5581 ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG 5641 GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC CGGATTCAGT 5701 CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG 5761 TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG 5821 GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT 5881 TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC 5941 AGAATTGGTT AATTGGTTGT AACACTGGCA GAGCATTACG CTGACTTGAC GGGACGGCGC 6001 AAGCTCATGA CCAAAATCCC TTAACGTGAG TTACGCGTCG TTCCACTGAG CGTCAGACCC 6061 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT 6121 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC 6181 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT 6241 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT 6301 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA 6361 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC 6421 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG 6481 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT 6541 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC 6601 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG 6661 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC 6721 TTTTGCTCAC ATGTT //