LOCUS pGL2399 7917 bp DNA circular UNA 05-Oct-2010 DEFINITION Invitrogen MS/GW donor vector 221 FEATURES Location/Qualifiers Vector join(1938..7917,1..1282) /origin="Mutated diCre with New CRK3 UTRs (7,075bpcircular)Target vector: from NotI cut at 1553^1554 toBamHI cut at 1526^1527, 7,048bpOrientation: originalPlusstrand 5': no changePlus strand 3': no changeMinusstrand 5': no changeMinus strand 3': no change" misc_feature 76..>82 /label="attR4 recombination site" misc_recomb 76..96 /label="attB4" misc_feature <83..96 /feature_type="Gateway sequence" /label="attB4" Site join(100..107,104^105) /site_type="restriction site" /note="Name: PacI" /note="Pattern: ttaattaa" /note="cut_0_on_positive_strand: 34^35" /note="cut_0_on_negative_strand: 32^33" /note="inhibited_by: " /note="site_type: other" /note="restriction site" /label="PacI" 5'UTR 108..361 /label="pRib 5' SS UTR" misc_feature 363..>368 /feature_type="Gateway sequence" /label="attB1r" misc_recomb 363..382 /label="attB1" CDS <369..385 /label="attP1" /label="attL1" 5'UTR 396..590 /label="Actin 5' UTR" SAS 517^518 /label="Splice Leader Site" CDS 591..623 /cds_type="ORF" misc_feature 624..935 /feature_type="Rapamycin Binding Domain" /label="FKBP12" Linker 936..980 misc_feature 981..1106 /feature_type="Cre Recombinase Dimer" /label="Cre59" 3'UTR join(<1938..1946,1107..>1282) /label="Actin 3'UTR" Site join(1282..>1282,complement(<1283..>1286),1282^1283,complement(1282^1283)) /site_type="restriction site" /note="Name: BamHI" /note="Pattern: ggatcc" /note="cut_0_on_positive_strand: 1526^1527" /note="cut_0_on_negative_strand: 1530^1531" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label="BamHI" Site join(<1283..>1286,<1287..1287,1282^1283) /site_type="restriction site" /note="Name: BamHI" /note="Pattern: ggatcc" /note="cut_0_on_positive_strand: 5^6" /note="cut_0_on_negative_strand: 9^10" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label="BamHI" misc_feature 1283..1937 /feature_type="Insertion" /origin="Actin PAS 3'UTR (672bp linear)Fragment: fromBamHI cut at 5^6 to NotI cut at 662^663,657bpOrientation: originalPlus strand 5': no changePlusstrand 3': no changeMinus strand 5': no changeMinusstrand 3': no change" /label="Insert" 3'UTR <1288..>1935 /label="3' UTR Actin (mexicana)" polyA_signal 1737^1738 Site join(1936..>1937,complement(1938..>1941),1937^1938,complement(1937^1938)) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="cut_0_on_positive_strand: 656^657" /note="cut_0_on_negative_strand: 660^661" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label="NotI" Site join(<1938..>1941,<1942..1943,1937^1938) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="cut_0_on_positive_strand: 1547^1548" /note="cut_0_on_negative_strand: 1551^1552" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label="NotI" 5'UTR 1947..2144 /label="Beta Tubulin 5'UTR" SAS 2070 /label="Splice Leader Site" CDS 2145..2177 /label="ORF" misc_feature 2178..2454 /feature_type="Rapamycin Binding Domain" /label="FRB" Linker 2455..2489 misc_feature 2490..3344 /feature_type="Cre Recombinase Dimer" /label="Cre60" 3'UTR 3345..3542 /label="Beta Tubulin 3'UTR" 5'UTR 3564..3863 /label="BLAr 5'UTR" CDS 3864..4259 /label="BLAr" 3'UTR 4260..4559 /label="BLAr 3'UTR" CDS 4575..>4586 /label="attP2" /label="attl2" misc_recomb complement(4580..4600) /label="attB2" misc_feature <4587..4600 /feature_type="Gateway sequence" /label="attB2r" 3'UTR 4603..5558 /label="pRib 3' SS UTR" Site join(5559..5566,5562^5563) /site_type="restriction site" /note="Name: PmeI" /note="Pattern: gtttaaac" /note="cut_0_on_positive_strand: 371^372" /note="cut_0_on_negative_strand: 371^372" /note="inhibited_by: " /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label="PmeI" misc_feature 5568..>5575 /feature_type="Gateway sequence" /label="attB3" misc_recomb complement(5569..5589) /label="attB3" misc_feature <5576..5589 /label="attR3 recombination site" CDS 6192..7052 /label="AmpR gene" CDS 7197..7870 /label="pUC ori" ORIGIN 1 CCTTTGAGTG AGCTGATACC AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG 61 ATTACGCCAA GCTATCAACT TTGTATAGAA AAGTTGCCCT TAATTAATGA CGAACAACTG 121 CCCTATCAGC TTGTGATGGC CGTGTAGTGG ACTGCCATGG CGTTGACGGG AGCGGGGGAT 181 TAGGGTTCGA TTCCGGAGAG GGAGCCTGAG AAATAGCTAC CACTTCTACG GAGGGCAGCA 241 GGCGCGCAAA TTGCCCAATG TCAAAACAAA ACGATGAGGC AGCGGAAAGG AATAGAGTTG 301 TCAGTCCATT TGGATTGTCA TTTCAATGGG GGATATTTAA ACCCATCCAA TATCGAGTAA 361 CGAAGTTTGT ACAAAAAAGC AGGCTTGCCC TCGAGGTTTT CGTCGCGCAT CGGTGTAGGT 421 CGAATACCCT TGCCTTTCAG TGCGGGGTTT TTTCCTATAA AGCATTTATT GTCTCGACGC 481 TTCTCCTGCT TGGTTCTCTC TCGCACTTGC ATGACAGATT TCAAGATATC TTGCCTGTAC 541 ACTCGTCATT AAAAACAGCT TATTCCCGGT TATTTTTCTC AACGAAGAAA ATGGCCCCTA 601 AGAAGAAGAG AAAGGTGTCA AGAGGAGTGC AGGTGGAAAC CATCTCCCCA GGAGACGGGC 661 GCACCTTCCC CAAGCGCGGC CAGACCTGCG TGGTGCACTA CACCGGGATG CTTGAAGATG 721 GAAAGAAATT TGATTCCTCC AGGGACAGAA ACAAGCCCTT TAAGTTTATG CTAGGCAAGC 781 AGGAGGTGAT CCGAGGCTGG GAAGAAGGGG TTGCCCAGAT GAGTGTGGGT CAGAGAGCCA 841 AACTGACTAT ATCTCCAGAT TATGCCTATG GTGCCACTGG GCACCCAGGC ATCATCCCAC 901 CACATGCCAC TCTCGTCTTC GATGTGGAGC TTCTAAAACT GGAAGCTAGC CCCAGCAACC 961 CCGGCGCCAG CAACGGCTCC ACCTCTGATG AAGTCAGGAA GAACCTGATG GACATGTTCA 1021 GGGACAGGCA GGCCTTCTCT GAACACACCT GGAAGATGCT CCTGTCTGTG TGCAGATCCT 1081 GGGCTGCCTG GTGCAAGCTG AACTGAAGGT CCTCACCCGA CTGGGCAGCT GGAGTGCAGA 1141 GAACTTTGCG CGACCGGAAG ACGCTAGTGA GATTGGGCAC AGTGGAGCTA GCGTGCGCGC 1201 GGTAGCTTTG GCAGCACTGG GATTTTGTCC TCTGTACTGC CTGGCTCTCG TGTTTATTTT 1261 TGGTTAGAGT GTGCGTTGCT CGGATCCCTG CCTGGTTCTC GTGCTTATTT TTGGTTAGAT 1321 GGTGCGTTGT TCTACTTTCA ATCATCGTTT TGTTTTGTTT TTCATATCTG TTTTCCAAAG 1381 AACTCTGATT GTCTTCATCC ACCCGGTTTT CCCCGCCTGG TCTCGCTTTG AGATTGTTTT 1441 TATTCCTCTC CGGTTTTTCC CCATTGGGAT AAAAGTGCGT CCCGTGTGTC TACCCCTCCA 1501 GTCCCCACAA GCGGCTCAGA TGTACAGAGC ACCTGCTCTT CTCTGACGTG CACTTTTTGC 1561 TCTTGCAGTC TTGCGCGGCC TCTAGTCTCC CCTGCCACGC ACGGATGCTG ACGCCAAGCA 1621 GCATTCTGCG CAGGAAAGCG TGGTATCCCG ACGAGGCAGA TCGCGGGCTC GCATGTACCG 1681 GCGACGGACA TCCTACACAC ATTATCCTCA TGTAGAACCT CAAAGCAAGG GAAGAAGAAA 1741 AGGGTTGACA CGTTGTTATG TTTTTTTTTG TGCCTGGGAG TAAGCGGGCG CCGATCTTCA 1801 AGTATCCTCT CAATTTCACT GGCAGGGCGC GAAGTGCGCG GGCTCCTCGC GTGCCGCCGC 1861 AATGTCAGTC GCGGCTCTAC CAGTGCTACA GGGATGTCGG GTGGTGTCGT TGATGATTTG 1921 GCATCAAGTG CACGCGCGGC CGCTTTCTTC CATCTGCACT CGCTCTGCGC TTCGTTTTCT 1981 TCTTCCCTTG CGCGCGCGTG ACGCCGCGCT CCTCACCTGC GCGCCCCGCC CCGACTTGGG 2041 TCCTCTCGTG CTGCAACGGA CGCCGACAGC TTCCTTCCAC GCATTTCGTG CCTCCGCACC 2101 GTTCTCCACC GCCAGCGCAG ACTTGTACAT ACGCACACAC CACCATGGCC CCTAAGAAGA 2161 AGAGAAAGGT GTCAAGAATC CTCTGGCATG AGATGTGGCA TGAAGGCCTG GAAGAGGCAT 2221 CTCGTTTGTA CTTTGGGGAA AGGAACGTGA AAGGCATGTT TGAGGTGCTG GAGCCCTTGC 2281 ATGCTATGAT GGAACGGGGC CCCCAGACTC TGAAGGAAAC ATCCTTTAAT CAGGCCTATG 2341 GTCGAGATTT AATGGAGGCC CAAGAGTGGT GCAGGAAGTA CATGAAATCA GGGAATGTCA 2401 AGGACCTCCT CCAAGCCTGG GACCTCTATT ATCATGTGTT CCGACGAATC TCAGCTAGCC 2461 CCAGCAACCC CGGCGCCAGC AACGGCTCCA ACAGGAAATG GTTCCCTGCT GAACCTGAGG 2521 ATGTGAGGGA CTACCTCCTG TACCTGCAAG CCAGAGGCCT GGCTGTGAAG ACCATCCAAC 2581 AGCACCTGGG CCAGCTCAAC ATGCTGCACA GGAGGTCTGG CCTGCCTCGC CCTTCTGACT 2641 CCAATGCTGT GTCCCTGGTG ATGAGGAGAA TCAGAAAGGA GAATGTGGAT GCTGGGGAGA 2701 GAGCCAAGCA GGCCCTGGCC TTTGAACGCA CTGACTTTGA CCAAGTCAGA TCCCTGATGG 2761 AGAACTCTGA CAGATGCCAG GACATCAGGA ACCTGGCCTT CCTGGGCATT GCCTACAACA 2821 CCCTGCTGCG CATTGCCGAA ATTGCCAGAA TCAGAGTGAA GGACATCTCC CGCACCGATG 2881 GTGGGAGAAT GCTGATCCAC ATTGGCAGGA CCAAGACCCT GGTGTCCACA GCTGGTGTGG 2941 AGAAGGCCCT GTCCCTGGGG GTTACCAAGC TGGTGGAGAG ATGGATCTCT GTGTCTGGTG 3001 TGGCTGATGA CCCCAACAAC TACCTGTTCT GCCGGGTCAG AAAGAATGGT GTGGCTGCCC 3061 CTTCTGCCAC CTCCCAACTG TCCACCCGAG CCCTGGAAGG GATCTTTGAG GCCACCCACC 3121 GCCTGATCTA TGGTGCCAAG GATGACTCTG GGCAGAGATA CCTGGCCTGG TCTGGCCACT 3181 CTGCCAGAGT GGGTGCTGCC AGGGACATGG CCAGGGCTGG TGTGTCCATC CCTGAAATCA 3241 TGCAGGCTGG TGGCTGGACC AATGTGAACA TTGTGATGAA CTACATCAGA AACCTGGACT 3301 CTGAGACTGG GGCCATGGTG AGGCTGCTAG AGGATGGGGA CTGAAGTGTG CGTGAGTGTG 3361 CGCGTGCGTG TTGGTGCGCA CCGCGCTCGC TGTCCCCGCG GCGCACGCAC ACCTCCCTCG 3421 CTCTCTCCCC CTCTTTCCGT GCCTTCTTTG TCTCCCTTCC TGTACGCTAT TTTGGTTTCT 3481 TGTGCTTGCT TTATCTTGTG GGCGGGTGTG GGCCCGACCT GCACGTCTGT GACGGAGTGA 3541 CGTCTAGAGA GCTCGGGTCG ACCACACGTA CACACGTACA CACACACGTG CGCATGCTTG 3601 ACGCATACGC GACGAATTCG AAAGCTCACC TCATTCCTCC CTCCTCACAC CATCATCGGC 3661 ATCCATAGAG ACACGTGCGC GTAGAACGAT ACAGCTCACG CGCACAGAGA GGCGTGTTCT 3721 GGTCGTGCGC ATCATCGGCA CAGCACTGGC GGACGAAACT CGCACACAGG CACGCCGCCT 3781 CCTTTCACCC GTCATAGATA GTTGAATTAG ACGCCCTCCT CCTCCCTCAT CATCGCCGTC 3841 GTCATCCGGG TCCGAGCACT AGTATGGCCA AGCCTTTGTC TCAAGAAGAA TCCACCCTCA 3901 TTGAAAGAGC AACGGCTACA ATCAACAGCA TCCCCATCTC TGAAGACTAC AGCGTCGCCA 3961 GCGCAGCTCT CTCTAGCGAC GGCCGCATCT TCACTGGTGT CAATGTATAT CATTTTACTG 4021 GGGGACCTTG TGCAGAACTC GTGGTGCTGG GCACTGCTGC TGCTGCGGCA GCTGGCAACC 4081 TGACTTGTAT CGTCGCGATC GGAAATGAGA ACAGGGGCAT CTTGAGCCCC TGCGGACGGT 4141 GCCGACAGGT GCTTCTCGAT CTGCATCCTG GGATCAAAGC CATAGTGAAG GACAGTGATG 4201 GACAGCCGAC GGCAGTTGGG ATTCGTGAAT TGCTGCCCTC TGGTTATGTG TGGGAGGGCT 4261 AAAGATCTTC TAGTGATCCC GCCCGCACCC GCGCGCGCTC AGGCCGCGTG TCGTCGTGCT 4321 CTTTCCATTT TTTTTTTGTG TGTGGTGGTG ATTTGAGCTG CTCTCCGTTG TGTGCTGGGG 4381 ACCCTCCTTC CCTCGATCTC CTCGTGCGGG GTCTCCGACG CGCAGACGCG GTGCGTGCGA 4441 GCGTGCACGT GCTGTCGCCG TGGCTGTAGT TGCGAGCGGA GAGAGAGAGA GAGGGAGGGA 4501 GGGAGGGCAG AGGGCAGAGG ACATGCGGGT GGGAACGTGC ACCGGCCTCG TCTCACGCAG 4561 GCCCGGGCGG TACCCTACCC AGCTTTCTTG TACAAAGTGG GGCGACTAGA CCGTAACGCC 4621 TTTTCAACTC ACGGCCTCTA GGAATGAAGG AGGGTAGTTC GGGGGAGAAC GTACTGGGGC 4681 GTCAGAGGTG AAATTCTTAG ACCGCACCAA GACGAACTAC AGCGAAGGCA TTCTTCAAGG 4741 ATACCTTCCT CAATCAAGAA CCAAAGTGTG GAGATCGAAG ATGATTAGAG ACCATTGTAG 4801 TCCACACTGC AAACGATGAC ACCCATGAAT TGGGGATCTT ATGGGCCGGC CTGCGGCAGG 4861 GTTTACCCTG TGTCCAGCAC CGCGCCCGCT TTTACCACCT TACGTATCCT TTCTATTCGG 4921 CCTTTACCGG CCACCCACGG GAATATCCTC AGCACGTTTT CTGTTTTTTC ACGCGAAAGC 4981 TTTGAGGTTA CAGTCTCAGG GGGGAGTACG TTCGCAAGAG TGAAACTTAA AGAAATTGAC 5041 GGAATGGCAC CACAAGACGT GGAGCGTGCG GTTTAATTTG ACTCAACACG GGGAACTTTA 5101 CCAGATCCGG ACAGGATGAG GATTGACAGA TTGAGTGTTC TTTCTCGATT CCCTGAATGG 5161 TGGTGCATGG CCGCTTTTGG TCGGTGGAGT GATTTGTTTG GTTGATTCCG TCAACGGACG 5221 AGATCCAAGC TGCCCAGTAG AATTCAGAAT TGCCCATAGA ATAGCAAACT CATCGGCGGG 5281 TTTTACCCAA CGGTGGGCTG CATTCGGTCG AATTCTTCTC TGCGGGATTC CTTTGTAATT 5341 GCACAAGGTG AAATTTTGGG CAACAGCAGG TCTGTGATGC TCCTCAATGT TCTGGGCGAC 5401 ACGCGCACTA CAATGTCAGT GAGAACAAGA AAAACGACTT TTGTCGAACC TACTTGATCA 5461 AAAGAGTGGG GAAACCCCGG AATCACATAG ACCCACTTGG GACCGAGGAT TGCAATTATT 5521 GGTCGCGCAA CGAGGAATGT CTCGTAGGCG CAGCTCATGT TTAAACCGCA ACTTTATTAT 5581 ACATAGTTGA TAATTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG 5641 TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG 5701 AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGCCTGA 5761 TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATGG TGCACTCTCA 5821 GTGCAATCTG CTCTGATGCC GCATAGTTAA GCCAGCCCCG ACACCCGCCA ACACCCGCTG 5881 ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT 5941 CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGACGAAAGG 6001 GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT 6061 CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC 6121 ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA 6181 AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT 6241 TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC 6301 AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA 6361 GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG 6421 CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC 6481 AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG 6541 TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC 6601 TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG 6661 TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG 6721 ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC 6781 TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC 6841 CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG 6901 AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGTCAGA TGGTAAGCCC TCCCGTATCG 6961 TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG 7021 AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC 7081 TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG 7141 ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG 7201 TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC 7261 AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC 7321 TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT 7381 AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC 7441 TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT 7501 CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC 7561 AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG 7621 AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG 7681 GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG 7741 TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA 7801 GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGTCTTT TGCTGGCCTT 7861 TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCG //